Review




Structured Review

Proteintech e cad
E Cad, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 3247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cad/E-cadherin+Polyclonal+antibody/pm41803986-78-11-14
Average 96 stars, based on 3247 article reviews
e cad - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Immunohistochemistry:

Article Title: FBL promotes hepatocellular carcinoma tumorigenesis and progression by recruiting YY1 to enhance CAD gene expression
Article Snippet: .. The antibodies used for IHC were anti-FBL (Abcam, AB166630, 1:200), anti-CAD (Proteintech, 16617-AP, 1:200), anti-RBPJ (Proteintech, 14613-AP, 1:100), and anti-Ki67 (Abcam, ab16667.1:100) antibodies. ..

Article Title: De novo pyrimidine biosynthetic complexes support cancer cell proliferation and ferroptosis defence.
Article Snippet: De novo pyrimidine biosynthesis is achieved by cytosolic carbamoyl-phosphate synthetase II, aspartate transcarbamylase and dihydroorotase (CAD) and uridine 5′-monophosphate synthase (UMPS), and mitochondrial dihydroorotate dehydrogenase (DHODH).. However, how these enzymes are orchestrated remains enigmatical.. Here we show that cytosolic glutamate oxaloacetate transaminase 1 clusters with CAD and UMPS, and this complex then connects with DHODH, which is mediated by the mitochondrial outer membrane protein voltage-dependent anion-selective channel protein 3.

Incubation:

Article Title: p53 activation enhances the sensitivity of non-small cell lung cancer to the combination of SH003 and docetaxel by inhibiting de novo pyrimidine synthesis
Article Snippet: .. They were then incubated with the following primary antibodies overnight at 4 °C: anti-CAD, DHODH, UMPS, UPP1, and UCK2 (Proteintech, USA), and anti-PARP, p53, p-p53, γ-H2AX, H2AX, and p21 (Cell Signaling, USA). .. The blots were incubated with secondary antibodies, followed by exposure using Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific, USA).

Article Title: p53 activation enhances the sensitivity of non-small cell lung cancer to the combination of SH003 and docetaxel by inhibiting de novo pyrimidine synthesis.
Article Snippet: .. They were then incubated with the following primary antibodies overnight at 4 °C: anti-CAD, DHODH, UMPS, UPP1, and UCK2 (Proteintech, USA), and anti-PARP, p53, p-p53, γ-H2AX, H2AX, and p21 (Cell Signaling, USA). .. The blots were incubated with secondary antibodies, followed by exposure using Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific, USA).

Western Blot:

Article Title: Redistribution of defective mitochondria-mediated dihydroorotate dehydrogenase imparts 5-fluorouracil resistance in colorectal cancer
Article Snippet: .. The primary antibodies used in immunoblotting included anti-FATP4 (1:2,000, ab199719, Abcam), anti-FABP1 (1:5,000, ab171739, Abcam), anti-CD36 (1:1,000, ab252922, Abcam), anti-FASN (1:2,000, ab128856, Abcam), anti-CPT1A (1:2,000, 15184-1-AP, Proteintech), anti-CPT2 (1:2,000, 26555-1-AP, Proteintech), anti-ACOX1 (1:4,000, 10957-1-AP, Proteintech), anti-caspase-3 (1:1,000, 9662, Cell Signaling), anti-cleaved caspase-3 (1:1,000, 9661, Cell Signaling), anti-SLC7A11 (1:4,000, ab175186, Abcam), anti-GPX4 (1:2,000, ab125066, Abcam), FSP1 (1:2,000, ab197896, Abcam), anti-GCH1 (1:2,000, ab236387, Abcam), anti-DHODH (1:1,000, ab246901, Abcam), anti-NDUFB8 (1:5,000, ab192878, Abcam), anti-SDHB (1:50,000, ab175225, Abcam), anti-UQCRC2 (1:2,000, 14742-1-AP, Proteintech), anti-MTCO1 (1:1,000, ab14705, Abcam), anti-ATP5A (1:2,000, ab176569, Abcam), anti-CAD (1:500, 16617-1-AP, Proteintech), and anti-TS (1:2,000, 15047-1-AP, Proteintech). .. Additionally, β-Actin (1:10,000, 81115-1-RR, Proteintech), α-Tubulin (1:20,000, 66031-1-Ig, Proteintech), and VDAC (1:1,000, 10866-1-AP, Proteintech) were used as internal references.



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